Biotechnology and Genetic Modification: Fermentation, Enzymes, Insulin Production, Genetic Engineering and Cloning

1. A fermenter is used to grow bacteria that produce an enzyme. The oxygen supply fails but the stirrer, temperature control and nutrient supply continue.
   Which result is most likely if the bacteria are aerobic?

A enzyme production increases because oxygen denatures toxins
B enzyme production decreases because aerobic respiration is reduced
C enzyme production is unchanged because bacteria never need oxygen
D bacteria immediately convert all enzyme into ethanol

2. Which condition in an industrial fermenter helps enzymes in microorganisms work at their optimum rate?

A maintaining a suitable temperature
B stopping all mixing
C allowing contamination by wild bacteria
D removing all nutrients after inoculation

3. Why is a fermenter sterilised before microorganisms are added?

A to kill the desired microorganisms
B to prevent contamination by unwanted microorganisms
C to increase genetic variation in all bacteria
D to remove all oxygen permanently

4. Which feature of a fermenter helps distribute nutrients, oxygen and microorganisms evenly?

A stirrer
B cooling jacket only
C outlet tap only
D pH probe only

5. In a fermenter, the temperature begins to rise above the optimum for enzyme production.
   Which part is most directly used to correct this?

A cooling jacket
B air inlet
C nutrient inlet
D foam breaker

6. Which statement about aseptic technique in biotechnology is correct?

A it prevents contamination by unwanted microorganisms
B it increases the mutation rate in bacteria
C it prevents enzymes from working
D it removes all DNA from microorganisms

7. Which microorganism is commonly used in bread-making?

A yeast
B Plasmodium
C HIV
D phagocyte

8. What gas makes bread dough rise during yeast fermentation?

A oxygen
B nitrogen
C carbon dioxide
D sulfur dioxide

9. Which equation represents anaerobic respiration in yeast?

A glucose + oxygen → carbon dioxide + water
B glucose → ethanol + carbon dioxide
C carbon dioxide + water → glucose + oxygen
D glucose → lactic acid

10. Why is yeast useful in brewing?

A it converts sugars into ethanol and carbon dioxide
B it converts ethanol into starch and oxygen
C it digests protein into antibodies
D it produces insulin using haemoglobin

11. In brewing, yeast is kept without oxygen after initial growth.
    Why?

A to force aerobic respiration
B to encourage ethanol production by anaerobic respiration
C to stop all enzyme activity
D to make yeast cells become viruses

12. Which product is made using bacteria or fungi in a fermenter and is used to kill bacteria?

A insulin
B penicillin
C ethanol
D lactic acid only

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

13. Penicillin is produced by a fungus. Which condition is needed to maximise production?

A sterile conditions and controlled temperature
B high temperature that denatures fungal enzymes
C no nutrients and no water
D contamination by many bacterial species

14. Which statement about industrial fermentation is correct?

A microorganisms are used because they can produce useful products rapidly
B microorganisms are avoided because they cannot be controlled
C fermentation always means photosynthesis in plants
D fermentation never requires enzymes

15. Which substance is used as the substrate for amylase in industry?

A starch
B protein
C lipid
D cellulose only

16. Which enzyme is used to convert starch into sugars?

A amylase
B protease
C lipase
D lactase

17. Which enzyme is used to produce lactose-free milk?

A lactase
B cellulase
C maltase only
D pectinase

18. What does lactase break down lactose into?

A glucose and galactose
B amino acids
C fatty acids and glycerol
D starch and cellulose

19. Which enzyme is used to increase juice yield from fruit pulp?

A pectinase
B protease
C insulin
D DNA ligase

20. Why does pectinase increase fruit juice yield?

A it breaks down pectin in plant cell walls
B it digests glucose into amino acids
C it converts ethanol into carbon dioxide
D it kills all yeast by denaturing DNA

21. Biological washing powders often contain protease and lipase.
    Which stains are these enzymes best suited to remove?

A blood and fat stains
B rust and sand stains
C salt and mineral ion stains
D oxygen and nitrogen stains

22. Why must biological washing powders not usually be used at very high temperatures?

A enzymes may be denatured
B stains become immune to washing powder
C bacteria produce chlorophyll
D detergent turns into glucose

23. Which statement about immobilised enzymes is correct?

A they are attached to a support and can be reused
B they are destroyed after one reaction every time
C they are dissolved freely and lost with the product
D they are converted into substrates during reaction

24. Which advantage is most directly linked to immobilised lactase in lactose-free milk production?

A enzyme can be separated from product and reused
B enzyme becomes a hormone
C lactose becomes starch
D milk becomes genetically modified automatically

25. A column contains immobilised lactase. Milk passes through the column too quickly.
    What is most likely?

A less lactose is broken down because contact time is reduced
B all lactose is broken down faster because speed always increases digestion
C lactase becomes DNA ligase
D milk proteins are converted into glucose

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

26. Which enzyme cuts DNA at specific base sequences during genetic engineering?

A restriction enzyme
B lipase
C amylase
D lactase

27. Which enzyme joins DNA fragments together during genetic engineering?

A DNA ligase
B protease
C pectinase
D cellulase

28. Which structure is commonly used as a vector to transfer a human gene into bacteria?

A plasmid
B ribosome
C chloroplast only
D haemoglobin molecule

29. Which sequence best describes genetic engineering of bacteria to produce human insulin?

A cut human insulin gene → insert into plasmid → place plasmid into bacterium → culture bacteria
B culture bacteria → remove all DNA → add insulin protein → make plasmid later
C inject insulin into bacteria → bacteria copy insulin using lungs → extract glucose
D cut bacterial ribosomes → attach to human pancreas → produce starch

30. Why are plasmids useful in genetic engineering?

A they are small circular DNA molecules that can carry inserted genes
B they are enzymes that digest lipids
C they are chromosomes found only in viruses
D they are antibodies that bind to insulin

31. A bacterium receives a plasmid containing the human insulin gene.
    What must happen for insulin to be produced?

A the gene must be expressed by the bacterium
B the gene must be converted into a cell wall
C the plasmid must destroy all ribosomes
D the bacterium must become a human pancreas cell

32. Which statement about genetically engineered insulin is correct?

A it can be produced by bacteria containing the human insulin gene
B it is produced by boiling yeast in ethanol
C it is made by red blood cells after vaccination
D it is made by mosquitoes carrying Plasmodium

33. Which advantage of producing insulin by genetic engineering is most valid?

A large quantities of human insulin can be produced without relying on animal pancreases
B it makes insulin unnecessary for type 1 diabetes
C it prevents all mutations in bacteria
D it produces insulin only inside human red blood cells

34. Which possible concern is linked with genetically modified crops?

A inserted genes may spread to wild relatives
B crops cannot photosynthesise after modification
C all GM crops become viruses
D GM crops always produce no seeds

35. Which benefit could be gained from a crop genetically modified for pest resistance?

A reduced need for chemical insecticides
B increased eutrophication in every river
C permanent removal of all decomposers
D lower yield in every environment

36. A GM crop produces a toxin that kills a pest insect.
    Which risk must be considered?

A possible effects on non-target insects
B increased oxygen transport by haemoglobin
C complete prevention of meiosis in humans
D conversion of pesticide into insulin

37. Which statement about genetic engineering is correct?

A it involves changing an organism’s DNA by inserting, deleting or modifying genes
B it is always the same as natural selection
C it only changes an organism’s diet
D it cannot be used in microorganisms

38. Which statement correctly compares selective breeding and genetic engineering?

A selective breeding directly inserts a known gene; genetic engineering only chooses parents
B selective breeding chooses parents with desired traits; genetic engineering directly changes DNA
C both always produce identical clones by mitosis only
D neither can change inherited features

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

39. Which statement about clones is correct?

A clones are genetically identical organisms or cells produced from one parent
B clones are always produced by fertilisation between two parents
C clones always show maximum genetic variation
D clones contain no DNA

40. Which example is natural cloning in plants?

A runners producing new strawberry plants
B fertilisation inside an ovule
C cross-pollination by bees
D seed formation after meiosis

41. Which method can produce many genetically identical plants from a small piece of plant tissue?

A tissue culture
B random pollination
C cross-breeding only
D wind dispersal

42. Why are sterile conditions needed in plant tissue culture?

A to prevent contamination by microorganisms
B to stop plant cells from dividing
C to remove all nutrients from the growth medium
D to prevent mitosis permanently

43. Which substance is often added to tissue culture medium to control shoot and root development?

A plant hormones
B haemoglobin
C bile
D antibodies

44. Which disadvantage is associated with cloning a crop plant many times?

A low genetic variation may make all plants vulnerable to the same disease
B offspring become different species immediately
C all offspring lose chloroplasts
D photosynthesis becomes impossible in every clone

45. Which advantage of cloning plants is most valid?

A desirable characteristics can be copied quickly and reliably
B maximum genetic variation is produced
C all pests are permanently removed from ecosystems
D mutation rate becomes zero forever

46. In adult cell cloning of animals, which structure is removed from an egg cell before inserting a donor nucleus?

A nucleus
B cell membrane
C mitochondria only
D cytoplasm only

47. In adult cell cloning, the nucleus inserted into the egg cell comes from

A a body cell of the organism to be cloned
B a sperm cell from a different species
C a bacterial plasmid containing chlorophyll
D a virus with no genetic material

48. Which statement about the animal produced by adult cell cloning is correct?

A it is genetically identical to the nucleus donor
B it is genetically identical to the surrogate mother only
C it has no chromosomes
D it is produced by fusion of two gamete nuclei

49. Which sequence best describes adult cell cloning?

A remove egg nucleus → insert donor body-cell nucleus → stimulate division → implant embryo into surrogate
B fertilise egg with sperm → pollinate flower → form seed → germinate
C cut plasmid → add insulin gene → transform bacterium → produce hormone
D remove all DNA → boil embryo → inject antibiotics → form clone

50. Which statement is correct?

A biotechnology uses organisms or biological processes to make useful products
B genetic engineering always produces organisms with no genes
C fermentation can occur only in human lungs
D cloning always requires pollination and fertilisation

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

1. Answer: B
   A is wrong because oxygen is needed by aerobic bacteria for aerobic respiration.
   B is correct because if aerobic bacteria lose oxygen supply, aerobic respiration falls, so less energy is available for growth and enzyme production.
   C is wrong because many bacteria do need oxygen if they are aerobic.
   D is wrong because bacteria do not immediately convert all enzyme into ethanol.

2. Answer: A
   A is correct because a suitable temperature allows microbial enzymes to work near their optimum rate.
   B is wrong because stopping mixing reduces even distribution of nutrients and oxygen.
   C is wrong because contamination competes with or destroys the desired microorganisms.
   D is wrong because nutrients are needed for growth and product formation.

3. Answer: B
   A is wrong because sterilisation happens before adding the desired microorganisms.
   B is correct because sterilising the fermenter prevents contamination by unwanted microorganisms.
   C is wrong because sterilisation does not increase genetic variation.
   D is wrong because oxygen may be needed, especially for aerobic microorganisms.

4. Answer: A
   A is correct because the stirrer mixes the contents so nutrients, oxygen and microorganisms are evenly distributed.
   B is wrong because the cooling jacket controls temperature.
   C is wrong because the outlet tap removes product or culture.
   D is wrong because a pH probe monitors pH.

5. Answer: A
   A is correct because the cooling jacket removes excess heat and returns the fermenter to the optimum temperature.
   B is wrong because the air inlet supplies oxygen.
   C is wrong because the nutrient inlet adds nutrients.
   D is wrong because the foam breaker controls foam, not temperature directly.

6. Answer: A
   A is correct because aseptic technique prevents contamination by unwanted microorganisms.
   B is wrong because it is not designed to increase mutation rate.
   C is wrong because enzymes still work under aseptic conditions.
   D is wrong because it does not remove all DNA from microorganisms.

7. Answer: A
   A is correct because yeast is used in bread-making.
   B is wrong because Plasmodium causes malaria.
   C is wrong because HIV is a virus linked with AIDS.
   D is wrong because a phagocyte is a white blood cell, not a bread-making microorganism.

8. Answer: C
   A is wrong because oxygen is not the gas that makes dough rise.
   B is wrong because nitrogen is not produced by yeast fermentation.
   C is correct because carbon dioxide bubbles make bread dough rise.
   D is wrong because sulfur dioxide is not produced in bread-making fermentation.

9. Answer: B
   A is wrong because this is aerobic respiration.
   B is correct because anaerobic respiration in yeast produces ethanol and carbon dioxide.
   C is wrong because this is photosynthesis.
   D is wrong because glucose → lactic acid is anaerobic respiration in human muscles, not yeast.

10. Answer: A
    A is correct because yeast converts sugars into ethanol and carbon dioxide during anaerobic respiration.
    B is wrong because yeast does not convert ethanol into starch and oxygen.
    C is wrong because yeast does not digest protein into antibodies.
    D is wrong because insulin production by genetic engineering is not brewing.

11. Answer: B
    A is wrong because lack of oxygen prevents aerobic respiration.
    B is correct because anaerobic conditions encourage yeast to produce ethanol.
    C is wrong because enzyme activity continues in yeast fermentation.
    D is wrong because yeast cells do not become viruses.

12. Answer: B
    A is wrong because insulin controls blood glucose and is not used to kill bacteria.
    B is correct because penicillin is an antibiotic produced by a fungus and used to kill/inhibit bacteria.
    C is wrong because ethanol is used in alcoholic drinks and as a solvent/fuel, not as an antibiotic in this context.
    D is wrong because lactic acid is not the antibiotic product here.

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

13. Answer: A
    A is correct because sterile conditions and controlled temperature help the fungus grow and produce penicillin efficiently.
    B is wrong because high temperature may denature enzymes and reduce production.
    C is wrong because fungi need nutrients and water.
    D is wrong because contamination by bacteria may reduce yield or spoil the culture.

14. Answer: A
    A is correct because microorganisms reproduce quickly and can produce useful products rapidly in controlled conditions.
    B is wrong because microorganisms can be controlled in fermenters.
    C is wrong because fermentation is not photosynthesis.
    D is wrong because fermentation depends on enzyme-controlled reactions.

15. Answer: A
    A is correct because amylase acts on starch.
    B is wrong because protease acts on protein.
    C is wrong because lipase acts on lipid.
    D is wrong because cellulase acts on cellulose.

16. Answer: A
    A is correct because amylase converts starch into sugars such as maltose.
    B is wrong because protease digests protein.
    C is wrong because lipase digests lipids.
    D is wrong because lactase digests lactose.

17. Answer: A
    A is correct because lactase is used to produce lactose-free milk.
    B is wrong because cellulase digests cellulose.
    C is wrong because maltase digests maltose, not lactose.
    D is wrong because pectinase is used in fruit juice production.

18. Answer: A
    A is correct because lactase breaks lactose into glucose and galactose.
    B is wrong because proteases produce amino acids.
    C is wrong because lipases produce fatty acids and glycerol.
    D is wrong because lactose is not broken into starch and cellulose.

19. Answer: A
    A is correct because pectinase increases juice yield from fruit pulp.
    B is wrong because protease digests proteins.
    C is wrong because insulin is a hormone.
    D is wrong because DNA ligase joins DNA fragments.

20. Answer: A
    A is correct because pectinase breaks down pectin in plant cell walls, releasing more juice.
    B is wrong because glucose is not digested into amino acids.
    C is wrong because ethanol is not converted into carbon dioxide by pectinase.
    D is wrong because pectinase does not kill yeast by denaturing DNA.

21. Answer: A
    A is correct because protease digests protein stains such as blood, and lipase digests fat stains.
    B is wrong because enzymes in washing powder do not remove rust and sand in this way.
    C is wrong because salt and mineral ions are not enzyme-digested stains.
    D is wrong because oxygen and nitrogen are gases, not stains.

22. Answer: A
    A is correct because very high temperatures denature enzymes, changing their active sites.
    B is wrong because stains do not become immune.
    C is wrong because bacteria do not produce chlorophyll in washing powder.
    D is wrong because detergent does not turn into glucose.

23. Answer: A
    A is correct because immobilised enzymes are attached to a support and can be reused.
    B is wrong because enzymes are not necessarily destroyed after one reaction.
    C is wrong because immobilisation prevents the enzyme being lost with the product.
    D is wrong because enzymes are not converted into substrates.

24. Answer: A
    A is correct because immobilised lactase can be separated from the milk and reused, reducing cost and contamination.
    B is wrong because lactase does not become a hormone.
    C is wrong because lactose is broken into glucose and galactose, not starch.
    D is wrong because lactose-free milk production does not automatically genetically modify milk.

25. Answer: A
    A is correct because if milk flows too quickly, lactose has less contact time with lactase, so less is broken down.
    B is wrong because faster flow does not always mean more digestion; contact time matters.
    C is wrong because lactase does not become DNA ligase.
    D is wrong because milk proteins are not converted into glucose by lactase.

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

26. Answer: A
    A is correct because restriction enzymes cut DNA at specific base sequences.
    B is wrong because lipase digests lipids.
    C is wrong because amylase digests starch.
    D is wrong because lactase digests lactose.

27. Answer: A
    A is correct because DNA ligase joins DNA fragments together.
    B is wrong because protease digests protein.
    C is wrong because pectinase digests pectin.
    D is wrong because cellulase digests cellulose.

28. Answer: A
    A is correct because plasmids are commonly used as vectors to transfer genes into bacteria.
    B is wrong because ribosomes make proteins but are not vectors.
    C is wrong because chloroplasts are not the usual vector for bacteria.
    D is wrong because haemoglobin is a protein, not a DNA vector.

29. Answer: A
    A is correct because genetic engineering for insulin involves cutting out the human insulin gene, inserting it into a plasmid, putting the plasmid into bacteria and culturing them.
    B is wrong because adding insulin protein to bacteria will not make them produce insulin genetically.
    C is nonsense because bacteria do not copy insulin using lungs.
    D is wrong because ribosomes are not cut and attached to a pancreas to produce starch.

30. Answer: A
    A is correct because plasmids are small circular DNA molecules that can carry inserted genes.
    B is wrong because enzymes such as lipase digest lipids.
    C is wrong because plasmids are common in bacteria, not only viruses.
    D is wrong because antibodies are proteins, not plasmids.

31. Answer: A
    A is correct because the insulin gene must be expressed, meaning the bacterium uses it to make insulin protein.
    B is wrong because genes do not become cell walls.
    C is wrong because ribosomes are needed for protein synthesis.
    D is wrong because bacteria do not need to become pancreas cells to express an inserted gene.

32. Answer: A
    A is correct because bacteria containing the human insulin gene can produce human insulin.
    B is wrong because boiling yeast in ethanol does not make insulin.
    C is wrong because red blood cells do not make insulin after vaccination.
    D is wrong because mosquitoes carrying Plasmodium are linked with malaria, not insulin production.

33. Answer: A
    A is correct because genetic engineering allows large-scale production of human insulin without using animal pancreases.
    B is wrong because type 1 diabetes still usually needs insulin treatment.
    C is wrong because genetic engineering does not prevent all bacterial mutations.
    D is wrong because insulin is produced by engineered microorganisms, not human red blood cells.

34. Answer: A
    A is correct because one concern is that inserted genes may spread to wild relatives through cross-pollination.
    B is wrong because GM crops can still photosynthesise.
    C is wrong because GM crops do not all become viruses.
    D is wrong because GM crops do not always become seedless.

35. Answer: A
    A is correct because pest-resistant GM crops may reduce the need for chemical insecticides.
    B is wrong because this does not automatically increase eutrophication in every river.
    C is wrong because decomposers are not permanently removed.
    D is wrong because pest resistance often aims to increase yield, not lower it.

36. Answer: A
    A is correct because a toxin made by a GM crop may affect non-target insects as well as the pest.
    B is wrong because haemoglobin oxygen transport is unrelated.
    C is wrong because GM crop toxins do not prevent meiosis in humans completely.
    D is wrong because pesticide is not converted into insulin.

37. Answer: A
    A is correct because genetic engineering changes DNA by inserting, deleting or modifying genes.
    B is wrong because natural selection is differential survival and reproduction, not direct gene insertion.
    C is wrong because it changes DNA, not just diet.
    D is wrong because genetic engineering can be used in microorganisms.

38. Answer: B
    A is wrong because it reverses the definitions.
    B is correct because selective breeding chooses parents, while genetic engineering directly changes DNA.
    C is wrong because selective breeding and genetic engineering do not always produce clones.
    D is wrong because both can change inherited features.

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.

39. Answer: A
    A is correct because clones are genetically identical organisms or cells produced from one parent.
    B is wrong because fertilisation between two parents produces variation, not clones.
    C is wrong because clones have very low genetic variation.
    D is wrong because clones contain DNA.

40. Answer: A
    A is correct because runners producing new strawberry plants are natural cloning/asexual reproduction.
    B is wrong because fertilisation produces genetically varied offspring.
    C is wrong because cross-pollination is sexual reproduction.
    D is wrong because seed formation after meiosis/fertilisation is sexual reproduction.

41. Answer: A
    A is correct because tissue culture can produce many genetically identical plants from a small piece of tissue.
    B is wrong because random pollination produces variation.
    C is wrong because cross-breeding produces genetically varied offspring.
    D is wrong because wind dispersal spreads seeds; it does not clone tissue.

42. Answer: A
    A is correct because sterile conditions prevent contamination by microorganisms.
    B is wrong because tissue culture needs plant cells to divide.
    C is wrong because nutrients must be present in the growth medium.
    D is wrong because mitosis is needed to produce clones.

43. Answer: A
    A is correct because plant hormones such as auxins are used to control shoot and root development.
    B is wrong because haemoglobin transports oxygen.
    C is wrong because bile emulsifies fats.
    D is wrong because antibodies are immune proteins.

44. Answer: A
    A is correct because low genetic variation means all cloned plants may be vulnerable to the same disease.
    B is wrong because clones do not become different species immediately.
    C is wrong because clones of green plants do not automatically lose chloroplasts.
    D is wrong because photosynthesis does not become impossible in every clone.

45. Answer: A
    A is correct because cloning copies desirable characteristics quickly and reliably.
    B is wrong because cloning produces little variation.
    C is wrong because cloning does not permanently remove all pests.
    D is wrong because mutation rate does not become zero forever.

46. Answer: A
    A is correct because the nucleus is removed from the egg cell before inserting the donor nucleus.
    B is wrong because the cell membrane is kept.
    C is wrong because mitochondria are not the main structure removed in the basic cloning method.
    D is wrong because the cytoplasm is kept to support early development.

47. Answer: A
    A is correct because the donor nucleus comes from a body cell of the organism being cloned.
    B is wrong because a sperm cell is a gamete and from a different species would not be used this way.
    C is wrong because a bacterial plasmid with chlorophyll is nonsense here.
    D is wrong because viruses have genetic material, and this is not the donor nucleus source.

48. Answer: A
    A is correct because the clone is genetically identical to the organism that donated the nucleus.
    B is wrong because the surrogate mother only carries the embryo; she does not provide the nuclear DNA.
    C is wrong because the cloned animal has chromosomes from the donor nucleus.
    D is wrong because adult cell cloning does not involve fusion of two gamete nuclei.

49. Answer: A
    A is correct because adult cell cloning involves removing the egg nucleus, inserting a donor body-cell nucleus, stimulating division and implanting the embryo into a surrogate.
    B is wrong because that describes sexual reproduction and plant reproduction mixed together.
    C is wrong because that describes genetic engineering for insulin, not animal cloning.
    D is wrong because boiling an embryo and injecting antibiotics is not cloning.

50. Answer: A
    A is correct because biotechnology uses organisms or biological processes to make useful products.
    B is wrong because genetic engineering changes genes; it does not produce organisms with no genes.
    C is wrong because fermentation occurs in microorganisms such as yeast and bacteria, not only human lungs.
    D is wrong because cloning does not require pollination or fertilisation.

Written and Compiled By Sir Hunain Zia (AYLOTI), World Record Holder With 154 Total A Grades, 11 World Records and 7 Distinctions, Educate A Change.